Human CXCL5 Recombinant Rabbit Monoclonal Antibody [PSH18-71]
cat.: HA725362
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH18-71 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CXCL5 aa 37-114 (HA210887). |
| Positive control: | Recombinant Human CXCL5 protein (HA210887). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-70] to Human CXCL5 antibody (Capture) (HA725361) and Recombinant Human CXCL5 protein (HA210887) as the standard. The reference range value is 19.5-10,000 pg/mL. |
| Uniprot #: | SwissProt: P42830 Human |
| Alternative names: | AMCFII C-X-C motif chemokine 5 C-X-C motif chemokine ligand 5 Chemokine (C X C motif) ligand 5 chemokine (C-X-C motif) ligand 5 Cxcl5 CXCL5_HUMAN ENA 78 ENA-78 (8-78) ENA-78(1-78) ENA-78(9-78) ENA78 Epithelial derived neutrophil activating protein 78 Epithelial-derived neutrophil-activating protein 78 Lipopolysaccharide-induced CXC chemokine Neutrophil activating peptide ENA 78 Neutrophil activating protein 78 Neutrophil-activating peptide ENA-78 neutrophil-activating protein 78 SCYB5 Small inducible cytokine B5 small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78) small inducible cytokine subfamily B, member 5 Small-inducible cytokine B5 |
Images
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Fig1:
Sandwich ELISA analysis of human CXCL5 matched pair antibodies Capture: HA725361, Human CXCL5 Rabbit mAb [PSH18-70] Detector: HA725362, Human CXCL5 Rabbit mAb [PSH18-71] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725361) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL5 protein (HA210887) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725362, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL5 in HeLa cell culture supernatant and human plasma samples. Capture: HA725361, Human CXCL5 Rabbit mAb [PSH18-70] Detector: HA725362, Human CXCL5 Rabbit mAb [PSH18-71] The concentrations of CXCL5 were measured in duplicates, interpolated from the CXCL5 standard curve and corrected for sample dilution. Undiluted samples are HEK-293 cell culture supernatant 100% and human plasma 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL5 concentration was determined to be 6375 pg/ml in human plasma and undetectable in HEK-293 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.