Human CXCL16 Recombinant Rabbit Monoclonal Antibody [PSH21-54]
cat.: HA725363
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human CXCL16 aa 30-205 (HA211138). |
| Positive control: | Recombinant Human CXCL16 protein (HA211138). |
| Subcellular location: | Cell membrane, Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-55] to Human CXCL16 antibody (Detector) (HA725364) and Recombinant Human CXCL16 protein (HA211138) as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: Q9H2A7 Human |
| Alternative names: | C-X-C motif chemokine 16 Chemokine (C X C motif) ligand 16 Chemokine CXC motif ligand 16 CXC chemokine ligand 16 Cxcl16 CXCLG16 CXL16_HUMAN Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein SCYB16 Small inducible cytokine B16 precursor Small-inducible cytokine B16 SR-PSOX SRPSOX Transmembrane chemokine CXCL16 UNQ2759/PRO6714 |
Images
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Fig1:
Sandwich ELISA analysis of Human CXCL16 matched pair antibodies Capture: HA725363, Human CXCL16 Rabbit mAb [PSH21-54] Detector: HA725364, Human CXCL16 Rabbit mAb [PSH21-55] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725363) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CXCL16 protein (HA211138) starting from5,000 pg/ml to 0 pg/ml and detect antibody (HA725364, Biotin, 0.05µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native CXCL16 in HepG2 cell culture supernatant. Capture: HA725363, Human CXCL16 Rabbit mAb [PSH21-54] Detector: HA725364, Human CXCL16 Rabbit mAb [PSH21-55] The concentrations of CXCL16 were measured in duplicates, interpolated from the CXCL16 standard curve and corrected for sample dilution. Undiluted samples are HepG2 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL16 concentration was determined to be 1468 pg/ml in HepG2 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.