Biotin Conjugated Human VEGF Receptor 1 Recombinant Rabbit Monoclonal Antibody [PSH19-23]
cat.: HA725366B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH19-23 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human VEGF Receptor 1 aa 27-756 (HA211083). |
| Positive control: | Recombinant Human VEGF Receptor 1 protein (HA211083). |
| Subcellular location: | Cell membrane, Endosome; Secreted; Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-22] to Human VEGF Receptor 1 antibody (Capture) (HA725365) and Recombinant Human VEGF Receptor 1 protein (HA211083) as the standard. The reference range value is 39.1-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P17948 Human |
| Alternative names: | EC 2.7.10.1 FLT 1 FLT Flt-1 FLT1 Fms like tyrosine kinase 1 Fms related tyrosine kinase 1 Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor Fms-like tyrosine kinase 1 FRT Soluble VEGF receptor 1 14 Soluble VEGFR1 variant 2 Soluble VEGFR1 variant 21 Tyrosine protein kinase FRT Tyrosine protein kinase receptor FLT Tyrosine-protein kinase FRT Tyrosine-protein kinase receptor FLT Vascular endothelial growth factor receptor 1 Vascular endothelial growth factor vascular permeability factor receptor Vascular permeability factor receptor 1 Vascular permeability factor receptor VEGFR 1 VEGFR-1 VEGFR1 VGFR1_HUMAN |
Images
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Fig1:
Sandwich ELISA analysis of Human VEGF Receptor 1 matched pair antibodies Capture: HA725365, Human VEGF Receptor 1 Rabbit mAb [PSH19-22] Detector: HA725366, Human VEGF Receptor 1 Rabbit mAb [PSH19-23] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725365) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human VEGF Receptor 1 protein (HA211083) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725366, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native VEGFR1(FLT1) in human plasma samples. Capture: HA725365, Human VEGF Receptor 1 Rabbit mAb [PSH19-22] Detector: HA725366, Human VEGF Receptor 1 Rabbit mAb [PSH19-23] The concentrations of VEGFR1(FLT1) were measured in duplicates, interpolated from the VEGFR1(FLT1) standard curve and corrected for sample dilution. Undiluted samples are human plasma 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean VEGFR1(FLT1) concentration was determined to be 712.28 pg/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.