Biotin Conjugated Human TIMP1 Recombinant Rabbit Monoclonal Antibody [PSH18-79]
cat.: HA725368B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH18-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% ProClin300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TIMP1 aa 24-207 (HA211003). |
| Positive control: | Recombinant Human TIMP1 protein (HA211003). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH18-78] to Human TIMP1 antibody (Capture) (HA725367) and Recombinant Human TIMP1 protein (HA211003) as the standard. The reference range value is 78.1-20,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01033 Human |
| Alternative names: | Clgi Collagenase inhibitor Collagenase inhibitor, Human EPA EPO Erythroid Potentiating Activity Erythroid-potentiating activity Fibroblast collagenase inhibitor FLJ90373 HCI Human Collagenase Inhibitor Metalloproteinase inhibitor 1 Metalloproteinase inhibitor 1 precursor OTTHUMP00000023214 TIMP 1 TIMP TIMP metallopeptidase inhibitor 1 TIMP-1 Timp1 TIMP1 protein TIMP1_HUMAN Tissue Inhibitor of Metalloproteinase 1 Tissue inhibitor of metalloproteinases 1 Tissue inhibitor of metalloproteinases Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor |
Images
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Fig1:
Sandwich ELISA analysis of human TIMP1 matched pair antibodies Capture: HA725367, Human TIMP1 Rabbit mAb [PSH18-78] Detector: HA725368, Human TIMP1 Rabbit mAb [PSH18-79] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725367) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TIMP1 protein (HA211003) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725368, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native TIMP1 in human plasma samples. Capture: HA725367, Human TIMP1 Rabbit mAb [PSH18-78] Detector: HA725368, Human TIMP1 Rabbit mAb [PSH18-79] The concentrations of TIMP1 were measured in duplicates, interpolated from the TIMP1 standard curve and corrected for sample dilution. Undiluted samples are human plasma 2%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 272.4 ng/ml in human plasma. |
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Fig3:
Interpolated concentrations of native TIMP1 in HT-29 cell culture supernatant. Capture: HA725367, Human TIMP1 Rabbit mAb [PSH18-78] Detector: HA725368, Human TIMP1 Rabbit mAb [PSH18-79] The concentrations of TIMP1 were measured in duplicates, interpolated from the TIMP1 standard curve and corrected for sample dilution. Undiluted samples are HT-29 cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TIMP1 concentration was determined to be 16.1 ng/ml in HT-29 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.