Biotin Conjugated Human IL-32 Recombinant Rabbit Monoclonal Antibody [PSH20-79]
cat.: HA725370B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human IL-32 aa 1-131 (HA211338). |
| Positive control: | Recombinant Human IL-32 protein (HA211338). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-78] to Human IL-32 antibody (Capture) (HA725369) and Recombinant Human IL-32 protein (HA211338) as the standard. The reference range value is 31.3-8,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P24001 Human |
| Alternative names: | IL 32alpha IL 32beta IL 32delta IL 32gamma IL-32 IL32 IL32_HUMAN Interleukin 32 Interleukin 32 small Interleukin 32 theta Interleukin-32 interleukin-32 eta Natural killer cell transcript 4 Natural killer cells protein 4 NK 4 NK4 OTTHUMP00000236040 OTTHUMP00000236041 OTTHUMP00000236042 OTTHUMP00000236043 OTTHUMP00000236044 OTTHUMP00000236045 OTTHUMP00000236046 OTTHUMP00000236047 OTTHUMP00000236048 OTTHUMP00000236049 OTTHUMP00000236050 OTTHUMP00000236051 OTTHUMP00000236052 OTTHUMP00000236053 OTTHUMP00000236054 OTTHUMP00000241545 OTTHUMP00000241602 OTTHUMP00000241603 OTTHUMP00000241604 OTTHUMP00000241605 OTTHUMP00000241606 OTTHUMP00000241607 OTTHUMP00000241608 OTTHUMP00000241609 OTTHUMP00000241610 OTTHUMP00000241611 OTTHUMP00000241612 TAIF TAIFa TAIFb TAIFc TAIFd Tumor necrosis factor alpha-inducing factor |
Images
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Fig1:
Sandwich ELISA analysis of Human IL-32 matched pair antibodies Capture: HA725369, Human IL-32 Rabbit mAb [PSH20-78] Detector: HA725370, Human IL-32 Rabbit mAb [PSH20-79] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725369) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-32 protein (HA211338) starting from 7,000 pg/ml to 0 pg/ml and detect antibody (HA725370, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native IL-32 in NK-92 samples. Capture: HA725369, Human IL-32 Rabbit mAb [PSH20-78] Detector: HA725370, Human IL-32 Rabbit mAb [PSH20-79] The concentrations of IL-32 were measured in duplicates, interpolated from the IL-32 standard curve and corrected for sample dilution. Undiluted samples are NK-92. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-32 concentration was determined to be 17,271 pg/ml in NK-92. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.