Human FABP2 Recombinant Rabbit Monoclonal Antibody [PSH22-41]
cat.: HA725372
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-41 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FABP2 aa 1-132 (HA211411). |
| Positive control: | Recombinant Human FABP2 protein (HA211411). |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-40] to Human FABP2 antibody (Capture) (HA725371) and Recombinant Human FABP2 protein (HA211411) as the standard. The reference range value is 46.9-6,000 pg/mL. |
| Uniprot #: | SwissProt: P12104 Human |
| Alternative names: | FABP 2 FABP2 FABPI FABPI_HUMAN Fatty acid binding protein 2 intestinal Fatty acid binding protein Fatty acid binding protein intestinal Fatty acid-binding protein 2 Fatty acid-binding protein I FABP I-FABP IFABP intestinal intestinal FABP Intestinal fatty acid binding protein 2 Intestinal-type fatty acid-binding protein MGC133132 OTTHUMP00000163925 |
Images
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Fig1:
Sandwich ELISA analysis of Human FABP2 matched pair antibodies Capture: HA725371, Human FABP2 Rabbit mAb [PSH22-40] Detector: HA725372, Human FABP2 Rabbit mAb [PSH22-41] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725371) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FABP2 protein (HA211411) starting from 6,000 pg/ml to 0 pg/ml and detect antibody (HA725372, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native FABP2 in human serum samples. Capture: HA725371, Human FABP2 Rabbit mAb [PSH22-40] Detector: HA725372, Human FABP2 Rabbit mAb [PSH22-41] The concentrations of FABP2 were measured in duplicates, interpolated from the FABP2 standard curve and corrected for sample dilution. Undiluted samples are human serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration was determined to be 803.95 pg/ml in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.