Human IL1RL1 / ST2 Recombinant Rabbit Monoclonal Antibody [PSH19-92]
cat.: HA725375
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Cynomolgus monkey |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH19-92 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IL1RL1 / ST2 aa 19-328 (HA210713). |
| Positive control: | Recombinant Human IL1RL1 / ST2 protein (HA210713). |
| Subcellular location: | Cell membrane; Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH19-93] to Human IL1RL1 / ST2 antibody (Detector) (HA725376) and Recombinant Human IL1RL1 / ST2 protein (HA210713) as the standard. The reference range value is 39.1-5,000 pg/mL. |
| Uniprot #: | SwissProt: Q01638 Human |
| Alternative names: | DER-4 DER4 FIT 1 Growth stimulation expressed homolog of mouse growth stimulation-expressed Il1rl1 IL33R ILRL1_HUMAN Interleukin 1 receptor like 1 interleukin 1 receptor related protein Interleukin-1 receptor-like 1 Protein ST2 ST2 ST2L ST2V T1 T1 protein |
Images
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Fig1:
Sandwich ELISA analysis of Human IL1RL1 / ST2 matched pair antibodies Capture: HA725375, Human IL1RL1 / ST2 Rabbit mAb [PSH19-92] Detector: HA725376, Human IL1RL1 / ST2 Rabbit mAb [PSH19-93] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725375) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL1RL1 / ST2 protein (HA210713) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725376, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ST2 in human plasma samples. Capture: HA725375, Human IL1RL1 / ST2 Rabbit mAb [PSH19-92] Detector: HA725376, Human IL1RL1 / ST2 Rabbit mAb [PSH19-93] The concentrations of human ST2 were measured in duplicates, interpolated from the human ST2 standard curve and corrected for sample dilution. Undiluted samples are human plasma 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean human ST2 concentration was determined to be 4963.9 pg/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.