Human PLGF Recombinant Rabbit Monoclonal Antibody [PSH23-85]
cat.: HA725377
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-85 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PLGF aa 19-149 (HA211337). |
| Positive control: | Recombinant Human PLGF protein (HA211337). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-86] to Human PLGF (Detecor) (HA725378) and Recombinant Human PLGF protein (HA211337) as the standard. The reference range value is 39.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: P49763 Human |
| Alternative names: | D12S1900 Pgf PGFL PIGF Placenta growth factor Placental growth factor Placental growth factor, vascular endothelial growth factor related protein PlGF 2 PlGF PLGF_HUMAN PlGF2 SHGC 10760 |
Images
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Fig1:
Sandwich ELISA analysis of Human PLGF matched pair antibodies Capture: HA725377, Human PLGF Rabbit mAb [PSH23-85] Detector: HA725378, Human PLGF Rabbit mAb [PSH23-86] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725377) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PLGF protein (HA211337) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725378, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native PLGF in JAR cell culture supernatant. Capture: HA725377, Human PLGF Rabbit mAb [PSH23-85] Detector: HA725378, Human PLGF Rabbit mAb [PSH23-86] The concentrations of PLGF were measured in duplicates, interpolated from the PLGF standard curve and corrected for sample dilution. Undiluted samples are 100% JAR cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PLGF concentration was determined to be 1,457 pg/ml in JAR cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.