Human/Mouse MMP-2 Recombinant Rabbit Monoclonal Antibody [PSH22-04]
cat.: HA725379
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MMP-2 aa 28-267 (HA210660). |
| Positive control: | Recombinant Human MMP-2 protein (HA210660). |
| Subcellular location: | Secreted, extracellular space, extracellular matrixm Membrane, Nucleus; Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-05] to Human/Mouse MMP-2 antibody (Detector) (HA725380) and Recombinant Human MMP-2 protein (HA210660) as the standard. The reference range value is 78.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: P08253 Human | P33434 Mouse |
| Alternative names: | 72 kDa gelatinase 72kD type IV collagenase CLG 4 CLG 4A CLG4 CLG4A Collagenase Type 4 alpha Collagenase type IV A Gelatinase A Gelatinase alpha Gelatinase neutrophil Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) Matrix Metalloproteinase 2 Matrix metalloproteinase II Matrix metalloproteinase-2 MMP 2 MMP II MMP-2 MMP2 MMP2_HUMAN MONA Neutrophil gelatinase PEX TBE 1 TBE-1 |
Images
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Fig1:
Sandwich ELISA analysis of Human MMP-2 matched pair antibodies Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04] Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725379) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-2 protein (HA210660) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725380, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 9 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native MMP-2 in human serum samples. Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04] Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05] The concentrations of MMP-2 were measured in duplicates, interpolated from the MMP-2 standard curve and corrected for sample dilution. Undiluted samples are human serum 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-2 concentration was determined to be 169,094 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of native MMP-2 in mouse plasma samples. Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04] Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05] The concentrations of MMP-2 were measured in duplicates, interpolated from the MMP-2 standard curve and corrected for sample dilution. Undiluted samples are mouse plasma 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-2 concentration was determined to be 300,110 pg/ml in mouse plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.