Human/Mouse MMP-2 Recombinant Rabbit Monoclonal Antibody [PSH22-05]
cat.: HA725380
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MMP-2 aa 28-267 (HA210660). |
| Positive control: | Recombinant Human MMP-2 protein (HA210660). |
| Subcellular location: | Secreted, extracellular space, extracellular matrixm Membrane, Nucleus; Cytoplasm, Mitochondrion. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-04] to Human/Mouse MMP-2 antibody (Capture) (HA725379) and Recombinant Human MMP-2 protein (HA210660) as the standard. The reference range value is 78.1-20,000 pg/mL. |
| Uniprot #: | SwissProt: P08253 Human | P33434 Mouse |
| Alternative names: | 72 kDa gelatinase 72kD type IV collagenase CLG 4 CLG 4A CLG4 CLG4A Collagenase Type 4 alpha Collagenase type IV A Gelatinase A Gelatinase alpha Gelatinase neutrophil Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) Matrix Metalloproteinase 2 Matrix metalloproteinase II Matrix metalloproteinase-2 MMP 2 MMP II MMP-2 MMP2 MMP2_HUMAN MONA Neutrophil gelatinase PEX TBE 1 TBE-1 |
Images
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Fig1:
Sandwich ELISA analysis of Human MMP-2 matched pair antibodies Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04] Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725379) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-2 protein (HA210660) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725380, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native MMP-2 in human serum samples. Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04] Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05] The concentrations of MMP-2 were measured in duplicates, interpolated from the MMP-2 standard curve and corrected for sample dilution. Undiluted samples are human serum 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-2 concentration was determined to be 169,094 pg/ml in human serum. |
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Fig3:
Interpolated concentrations of native MMP-2 in mouse plasma samples. Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04] Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05] The concentrations of MMP-2 were measured in duplicates, interpolated from the MMP-2 standard curve and corrected for sample dilution. Undiluted samples are mouse plasma 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MMP-2 concentration was determined to be 300,110 pg/ml in mouse plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.