Biotin Conjugated Rat KIM-1/HAVCR1 Recombinant Rabbit Monoclonal Antibody [PSH20-50]
cat.: HA725382B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Rat KIM-1 / HAVCR1 aa 22-235 (HA211362). |
| Positive control: | Recombinant Rat KIM-1 / HAVCR1 protein (HA211362). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-49] to Rat KIM-1 / HAVCR1 antibody (Capture) (HA725381) and Recombinant Rat KIM-1 / HAVCR1 protein (HA211362) as the standard. The reference range value is 9.8-5,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O54947 Rat |
| Alternative names: | Hepatitis A virus cellular receptor 1 homolog HAVcr-1 Kidney injury molecule 1 (KIM-1) T cell immunoglobulin and mucin domain-containing protein 1 (TIMD-1) CD365 Havcr1 Kim1 |
Images
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Fig1:
Sandwich ELISA analysis of Rat KIM-1 / HAVCR1 matched pair antibodies Capture: HA725381, Rat KIM-1 / HAVCR1 Rabbit mAb [PSH20-49] Detector: HA725382, Rat KIM-1 / HAVCR1 Rabbit mAb [PSH20-50] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725381) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Rat KIM-1 / HAVCR1 protein (HA211362) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA725382, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 8 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Havcr1 in rat serum. Capture: HA725381, Rat KIM-1 / HAVCR1 Rabbit mAb [PSH20-49] Detector: HA725382, Rat KIM-1 / HAVCR1 Rabbit mAb [PSH20-50] The concentrations of Havcr1 were measured in duplicates, interpolated from the Havcr1 standard curve and corrected for sample dilution. Undiluted samples are rat serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Havcr1 concentration was determined to be 523 pg/ml in rat serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.