Human Galectin 3 Recombinant Rabbit Monoclonal Antibody [PSH20-53]
cat.: HA725385
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH20-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Human Galectin 3 aa 1-250 (HA211363). |
| Positive control: | Recombinant Human Galectin 3 protein (HA211363). |
| Subcellular location: | Cytoplasm, Nucleus, Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-54] to Human Galectin 3 antibody (Detector) (HA725386) and Recombinant Human Galectin 3 protein (HA211363) as the standard. The reference range value is 312.5-20,000 pg/mL. |
| Uniprot #: | SwissProt: P17931 Human |
| Alternative names: | 35 kDa lectin Carbohydrate binding protein 35 Carbohydrate-binding protein 35 CBP 35 CBP35 Gal-3 GAL3 Galactose-specific lectin 3 Galactoside-binding protein GALBP Galectin 3 internal gene,included Galectin-3 Galectin 3 Galectin3 GALIG GBP IgE binding protein IgE-binding protein L 31 L 34 L-31 L-34 galactoside-binding lectin L31 Laminin-binding protein Lectin L-29 Lectin, galactose binding, soluble 3 LEG3_HUMAN LGALS2 LGALS3 MAC 2 antigen Mac-2 Mac-2 antigen MAC2 Macrophage galactose-specific lectin MGC105387 |
Images
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Fig1:
Sandwich ELISA analysis of Human Galectin 3 matched pair antibodies Capture: HA725385, Human Galectin 3 Rabbit mAb [PSH20-53] Detector: HA725386, Human Galectin 3 Rabbit mAb [PSH20-54] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725385) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Galectin 3 protein (HA211363) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725386, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Galectin-3 in human serum samples and LNCaP cell culture supernatant. Capture: HA725385, Human Galectin 3 Rabbit mAb [PSH20-53] Detector: HA725386, Human Galectin 3 Rabbit mAb [PSH20-54] The concentrations of Galectin-3 were measured in duplicates, interpolated from the Galectin-3 standard curve and corrected for sample dilution. Undiluted samples are human serum 5% and LNCaP cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Galectin-3 concentration was determined to be 208 ng/ml in human serum and undetectable in LNCaP cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.