Biotin Conjugated Human ANGPTL4 Recombinant Rabbit Monoclonal Antibody [PSH20-15]
cat.: HA725390B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-15 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ANGPTL4 aa 166-406 (HA211387). |
| Positive control: | Recombinant Human ANGPTL4 protein (HA211387). |
| Subcellular location: | Secreted, extracellular space, extracellular matrix. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-14] to Human ANGPTL4 antibody (Capture) (HA725389) and Recombinant Human ANGPTL4 protein (HA211387) as the standard. The reference range value is 39.1-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q9BY76 Human |
| Alternative names: | Angiopoietin like 4 Angiopoietin related protein 4 Angiopoietin-like protein 4 Angiopoietin-related protein 4 ANGL4_HUMAN ANGPT L2 ANGPT L4 ANGPTL2 Angptl4 ARP4 Fasting induced adipose factor FIAF HARP Hepatic angiopoietin related protein Hepatic fibrinogen/angiopoietin related protein Hepatic fibrinogen/angiopoietin-related protein HFARP NL2 Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein PGAR pp1158 PPARG angiopoietin related protein PSEC0166 TGQTL UNQ171 Weakly similar to angiopoietin 1 [H.sapiens] |
Images
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Fig1:
Sandwich ELISA analysis of Human ANGPTL4 matched pair antibodies Capture: HA725389, Human ANGPTL4 Rabbit mAb [PSH20-14] Detector: HA725390, Human ANGPTL4 Rabbit mAb [PSH20-15] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725389) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant human ANGPTL4 (HA211387) protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725390, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ANGPTL4 in A549 cell culture supernatant and SW480 cell culture supernatant: Capture: HA725389, Human ANGPTL4 Rabbit mAb [PSH20-14] Detector: HA725390, Human ANGPTL4 Rabbit mAb [PSH20-15] The concentrations of ANGPTL4 were measured in duplicates, interpolated from the ANGPTL4 standard curve and corrected for sample dilution. Undiluted samples are A549 cell culture supernatant 100% and SW480 cell culture supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ANGPTL4 concentration was determined to be 1472 pg/ml in A549 cell culture supernatant and 395 pg/ml in SW480 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.