Human/Mouse MASP2 Recombinant Rabbit Monoclonal Antibody [PSH20-52]
cat.: HA725392
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rhesus monkey |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH20-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MASP2 aa 16-686. |
| Positive control: | Recombinant Human MASP2 protein. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-51] to Human/Mouse MASP2 antibody (Capture) (HA725391) and Recombinant Human/Mouse MASP2 protein as the standard. The reference range value is 39.1-10,000 pg/mL. |
| Uniprot #: | SwissProt: O00187 Human | Q91WP0 Mouse |
| Alternative names: | Mannan binding lectin serine peptidase 2 Mannan binding lectin serine protease 2 Mannose binding protein associated serine protease 2 MAP 19 MAP19 MASP 2 MBL associated plasma protein of 19 kD MBL associated serine protease 2 Small MBL associated protein sMAP |
Images
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Fig1:
Sandwich ELISA analysis of Human MASP2 matched pair antibodies Capture: HA725391, Human/Mouse MASP2 Rabbit mAb [PSH20-51] Detector: HA725392, Human/Mouse MASP2 Rabbit mAb [PSH20-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725391) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MASP2 protein starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725392, Biotin, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native MASP2 in human plasma samples. Capture: HA725391, Human/Mouse MASP2 Rabbit mAb [PSH20-51] Detector: HA725392, Human/Mouse MASP2 Rabbit mAb [PSH20-52] The concentrations of MASP2 were measured in duplicates, interpolated from the MASP2 standard curve and corrected for sample dilution. Undiluted samples are human plasma 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASP2 concentration was determined to be 29.29 ng/ml in human plasma. |
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Fig3:
Interpolated concentrations of native MAPS2 in mouse plasma samples. Capture: HA725391, Human/Mouse MASP2 Rabbit mAb [PSH20-51] Detector: HA725392, Human/Mouse MASP2 Rabbit mAb [PSH20-52] The concentrations of MAPS2 were measured in duplicates, interpolated from the MAPS2 standard curve and corrected for sample dilution. Undiluted samples are mouse plasma 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MAPS2 concentration was determined to be 369.42 pg/ml in mouse plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.