HRP Conjugated Human/Mouse MASP2 Recombinant Rabbit Monoclonal Antibody [PSH20-52]
cat.: HA725392H
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rhesus monkey |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH20-52 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MASP2 aa 16-686. |
| Positive control: | Recombinant Human MASP2 protein. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-51] to Human/Mouse MASP2 antibody (Capture) (HA725391) and Recombinant Human MASP2 protein as the standard. The reference range value is 78.13-10,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: O00187 Human | Q91WP0 Mouse |
| Alternative names: | Mannan binding lectin serine peptidase 2 Mannan binding lectin serine protease 2 Mannose binding protein associated serine protease 2 MAP 19 MAP19 MASP 2 MBL associated plasma protein of 19 kD MBL associated serine protease 2 Small MBL associated protein sMAP |
Images
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Fig1:
Sandwich ELISA analysis of Human MASP2 matched pair antibodies Capture: HA725391, Human/Mouse MASP2 Rabbit mAb [PSH20-51] Detector: HA725392, Human/Mouse MASP2 Rabbit mAb [PSH20-52] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725391) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MASP2 protein starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725392, HRP, 0.05 µg/ml) for 1 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.