Human Elafin Recombinant Rabbit Monoclonal Antibody [PSH20-06]
cat.: HA725393
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH20-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Elafin aa 23-117 (HA211336). |
| Positive control: | Recombinant Human Elafin protein (HA211336). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH20-07] to Human Elafin antibody (Detector) (HA725394) and Recombinant Human Elafin protein (HA211336) as the standard. The reference range value is 19.5-2,500 pg/mL. |
| Uniprot #: | SwissProt: P19957 Human |
| Alternative names: | Cementoin ELAF_HUMAN Elafin Elafin/Skalp Elastase Specific Inhibitor Elastase-specific inhibitor ES 1 ESI Peptidase inhibitor 3 Peptidase inhibitor 3 skin derived PI 3 PI-3 PI3 Pre elafin Protease inhibitor 3 skin derived Protease inhibitor 3 skin derived (SKALP) Protease inhibitor WAP3 SKALP Skin derived Anti leukoproteinase Skin-derived antileukoproteinase Trappin 2 WAP four disulfide core domain 14 WAP four disulfide core domain protein 14 WAP four-disulfide core domain protein 14 WAP3 WFDC14 |
Images
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Fig1:
Sandwich ELISA analysis of human Elafin matched pair antibodies Capture: HA725393, Human Elafin Rabbit mAb [PSH20-06] Detector: HA725394, Human Elafin Rabbit mAb [PSH20-07] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725393) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Elafin protein (HA211336) starting from 2,500 pg/ml to 0 pg/ml and detect antibody (HA725394, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Elafin in human plasma samples. Capture: HA725393, Human Elafin Rabbit mAb [PSH20-06] Detector: HA725394, Human Elafin Rabbit mAb [PSH20-07] The concentrations of Elafin were measured in duplicates, interpolated from the Elafin standard curve and corrected for sample dilution. Undiluted samples are human plasma 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Elafin concentration was determined to be 2186.6 pg/ml in human plasma. |
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Fig3:
Interpolated concentrations of native Elafin in A431 cell culture supernatant. Capture: HA725393, Human Elafin Rabbit mAb [PSH20-06] Detector: HA725394, Human Elafin Rabbit mAb [PSH20-07] The concentrations of Elafin were measured in duplicates, interpolated from the Elafin standard curve and corrected for sample dilution. Undiluted samples are A431 cell culture supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Elafin concentration was determined to be 819.5 pg/ml in A431 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.