Biotin Conjugated Human POMC Recombinant Rabbit Monoclonal Antibody [PSH22-66]
cat.: HA725396B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH22-66 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human POMC aa 26-267 (HA211433). |
| Positive control: | Recombinant Human POMC protein (HA211433). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-65] to Human POMC antibody (Capture) (HA725395) and Recombinant Human POMC protein (HA211433) as the standard. The reference range value is 15.6-1,000 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P01189 Human |
| Alternative names: | ACTH Adrenocorticotropic hormone Adrenocorticotropin alpha melanocyte stimulating hormone Alpha MSH Alpha-MSH beta endorphin Beta LPH beta melanocyte stimulating hormone Beta MSH Beta-LPH Beta-MSH CLIP COLI_HUMAN Corticotropin like intermediary peptide Corticotropin lipotropin Corticotropin-lipotropin Gamma MSH Gamma-LPH Gamma-MSH Lipotropin beta Lipotropin gamma LPH Melanotropin alpha Melanotropin Melanotropin beta Melanotropin gamma met enkephalin Met-enkephalin MSH NPP POC POMC pro ACTH endorphin Proopiomelanocortin Proopiomelanocortin preproprotein |
Images
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Fig1:
Sandwich ELISA analysis of Human POMC matched pair antibodies Capture: HA725395, Human POMC Rabbit mAb [PSH22-65] Detector: HA725396, Human POMC Rabbit mAb [PSH22-66] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725395) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted ACTH protein starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725396, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.