Mouse Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [PSH21-11]
cat.: HA725399
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH21-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Recombinant Mouse Lipocalin-2 aa 21-200 (HA211361). |
| Positive control: | Recombinant Mouse Lipocalin-2 protein (HA211361). |
| Subcellular location: | Secreted, Cytoplasmic granule lumen, Cytoplasmic vesicle lumen. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH21-12] to Mouse Lipocalin-2 antibody (Detector) (HA725400) and Recombinant Mouse Lipocalin-2 protein (HA211361) as the standard. The reference range value is 62.5-2,000 pg/mL. |
| Uniprot #: | SwissProt: P11672 Mouse |
| Alternative names: | 24p3 25 kDa alpha 2 microglobulin related subunit of MMP9 25 kDa alpha-2-microglobulin-related subunit of MMP-9 Alpha 2 microglobulin related protein HGNC:6526 HNL Lcn 2 Lcn2 Lipocalin-2 Migration stimulating factor inhibitor MSFI Neutrophil gelatinase associated lipocalin Neutrophil gelatinase associated lipocalin precursor Neutrophil gelatinase-associated lipocalin NGAL Oncogene 24p3 Oncogenic lipocalin 24p3 p25 Siderocalin siderocalin LCN2 SV40 induced 24P3 protein Uterocalin |
Images
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Fig1:
Sandwich ELISA analysis of Mouse Lipocalin-2 matched pair antibodies Capture: HA725399, Mouse Lipocalin-2 Rabbit mAb [PSH21-11] Detector: HA725400, Mouse Lipocalin-2 Rabbit mAb [PSH21-12] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725399) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse Lipocalin-2 protein (HA211361) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725400, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Lipocalin-2 in mouse plasma. Capture: HA725399, Mouse Lipocalin-2 Rabbit mAb [PSH21-11] Detector: HA725400, Mouse Lipocalin-2 Rabbit mAb [PSH21-12] The concentrations of mouse Lipocalin-2 measured in duplicate and interpolated from the mouse Lipocalin-2 standard curve and corrected for sample dilution. Undiluted samples are mouse plasma 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean mouse Lipocalin-2 concentration was determined to be 98.96 ng/ml in mouse plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.