Biotin Conjugated Human Tissue Factor Recombinant Rabbit Monoclonal Antibody [PSH22-46]
cat.: HA725402B
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det), ELISA |
| Clonality: | Monoclonal |
| Clone number: | PSH22-46 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Tissue Factor aa 33-251 (HA211432). |
| Positive control: | Recombinant Human Tissue Factor protein (HA211432). |
| Subcellular location: | Membrane; Screted. |
| Recommended Dilutions:
ELISA(Det) ELISA |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-45] to Human Tissue Factor antibody (Capture) (HA725401) and Recombinant Human Tissue Factor protein (HA211432) as the standard. The reference range value is 3.9-500 pg/mL. Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P13726 Human |
| Alternative names: | CD142 CD142 antigen Coagulation factor III (thromboplastin tissue factor) Coagulation factor III F3 FLJ17960 TF TF_HUMAN TFA Thromboplastin Tissue factor |
Images
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Fig1:
Sandwich ELISA analysis of Human Tissue Factor matched pair antibodies Capture: HA725401, Human Tissue Factor Rabbit mAb [PSH22-45] Detector: HA725402, Human Tissue Factor Rabbit mAb [PSH22-46] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725401) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Tissue Factor protein (HA211432) starting from 500 pg/ml to 0 pg/ml and detect antibody (HA725402, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native Tissue Factor in Hep G2 and 3T3-L1 lys samples based on a 1,000 µg/ml extract load. Capture: HA725401, Human Tissue Factor Rabbit mAb [PSH22-45] Detector: HA725402, Human Tissue Factor Rabbit mAb [PSH22-46] The concentrations of Tissue Factor were measured in duplicates, interpolated from the Tissue Factor standard curve and corrected for sample dilution. Undiluted samples are Hep G2 lys 0.5mg/ml and 3T3-L1 lys 1mg/ml. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Tissue Factor concentration was determined to be 170 pg/ml in Hep G2 lys and undetectable in 3T3-L1 lys. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.