Human Neutrophil Elastase Recombinant Rabbit Monoclonal Antibody [PSH22-06]
cat.: HA725417
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-06 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Neutrophil Elastase aa 28-267 (HA211430). |
| Positive control: | Recombinant Human Neutrophil Elastase protein (HA211430). |
| Subcellular location: | Cytoplasmic vesicle, phagosome. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-07] to Human Neutrophil Elastase antibody (Detector) (HA725418) and Recombinant Human Neutrophil Elastase protein (HA211430) as the standard. The reference range value is 7.8-4,000 pg/mL. |
| Uniprot #: | SwissProt: P08246 Human |
| Alternative names: | Bone marrow serine protease ELA2 ELANE Elastase 2 Elastase 2 neutrophil Elastase neutrophil expressed Elastase-2 ELNE_HUMAN GE Granulocyte derived elastase HLE HNE Human leukocyte elastase Leukocyte elastase Medullasin NE Neutrophil elastase PMN E PMN elastase Polymorphonuclear elastase SCN1 |
Images
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Fig1:
Sandwich ELISA analysis of Human Neutrophil Elastase matched pair antibodies Capture: HA725417, Human Neutrophil Elastase Rabbit mAb [PSH22-06] Detector: HA725418, Human Neutrophil Elastase Rabbit mAb [PSH22-07] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725417) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Neutrophil Elastase (HA211430) protein starting from 4,000 pg/ml to 0 pg/ml and detect antibody (HA725418, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native Neutrophil Elastase in U-937 cell extract and THP-1 cell extract: Capture: HA725417, Human Neutrophil Elastase Rabbit mAb [PSH22-06] Detector: HA725418, Human Neutrophil Elastase Rabbit mAb [PSH22-07] The concentrations of Neutrophil Elastase were measured in duplicates, interpolated from the Neutrophil Elastase standard curve and corrected for sample dilution. Undiluted samples are U-937 cell extract 5 µg/mL and THP-1 cell extract 5 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Neutrophil Elastase concentration was determined to be 3,018 pg/ml in U-937 cell culture supernatant and 336 pg/ml in THP-1 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.