Human ADAM17 Recombinant Rabbit Monoclonal Antibody [PSH28-41]
cat.: HA725421
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH28-41 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ADAM17 aa 18-671 (HA211625). |
| Positive control: | Recombinant Human ADAM17 protein (HA211625). |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH28-42] to Human ADAM17 (Detecor) (HA725422) and Recombinant Human ADAM17 protein (HA211625) as the standard. The reference range value is 156.3-5,000 pg/mL. |
| Uniprot #: | SwissProt: P78536 Human |
| Alternative names: | A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) A disintegrin and metalloproteinase domain 17 ADA17_HUMAN ADAM 17 ADAM metallopeptidase domain 17 ADAM17 ADAM17 protein CD 156b CD156b CD156b antigen CSVP Disintegrin and metalloproteinase domain-containing protein 17 MGC71942 NISBD NISBD1 Snake venom like protease Snake venom-like protease TACE TNF alpha convertase TNF alpha converting enzyme TNF-alpha convertase TNF-alpha-converting enzyme Tumor Necrosis Factor Alpha Converting Enzyme |
Images
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Fig1:
Sandwich ELISA analysis of Human ADAM17 protein matched pair antibodies Capture: HA725421, Human ADAM17 Rabbit mAb [PSH28-41] Detector: HA725422, Human ADAM17 Rabbit mAb [PSH28-42] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725421) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ADAM17 protein (HA211625) starting from 8,000 pg/mL to 0 pg/mL and detect antibody (HA725422, Biotin, 0.5 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ADAM17 in THP-1 cell extract and A549 cell extract Capture: HA725421, Human ADAM17 Rabbit mAb [PSH28-41] Detector: HA725422, Human ADAM17 Rabbit mAb [PSH28-42] The concentrations of ADAM17 were measured in duplicates, interpolated from the ADAM17 standard curve and corrected for sample dilution. Undiluted samples are THP-1 cell extract 2 mg/mL and A549 cell extract 2 mg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ADAM17 concentration was determined to be 42.26 ng/mL in THP-1 cell extract and 43.92 ng/mL in A549 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.