Human/Mouse Hemoglobin subunit alpha Recombinant Rabbit Monoclonal Antibody [PSH22-08]
cat.: HA725427
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH22-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Hemoglobin subunit alpha aa 1-142 (HA211410). |
| Positive control: | Recombinant Human Hemoglobin subunit alpha protein (HA211410). |
| Subcellular location: | Cytosol, cytosolic small ribosomal subunit, hemoglobin complex, blood microparticle, extracellular exosome, extracellular region, extracellular space, endocytic vesicle lumen, haptoglobin-hemoglobin complex, membrane. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH22-09] to Human/Mouse Hemoglobin subunit alpha antibody (Detector) (HA725428) and Recombinant Human Hemoglobin subunit alpha protein (HA211410) as the standard. The reference range value is 625-10,000 pg/mL. |
| Uniprot #: | SwissProt: P69905 Human | P01942 Mouse |
| Alternative names: | Alpha 1 globin Alpha globin Alpha one globin Alpha-globin HBA_HUMAN HBA1 HBA2 Hemoglobin alpha 1 Hemoglobin alpha 1 chain Hemoglobin alpha 1 globin chain Hemoglobin alpha 2 Hemoglobin alpha chain Hemoglobin subunit alpha MGC126895 MGC126897 |
Images
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Fig1:
Sandwich ELISA analysis of human HBA matched pair antibodies Capture: HA725427, Human/Mouse Hemoglobin subunit alpha Rabbit mAb [PSH22-08] Detector: HA725428, Human/Mouse Hemoglobin subunit alpha Rabbit mAb [PSH22-09] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725427) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Hemoglobin subunit alpha protein (HA211410) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725428, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native HBA in human serum. Capture: HA725427, Human/Mouse Hemoglobin subunit alpha Rabbit mAb [PSH22-08] Detector: HA725428, Human/Mouse Hemoglobin subunit alpha Rabbit mAb [PSH22-09] The concentrations of HBA were measured in duplicates, interpolated from the HBA standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and Ramos cell supernatant 0.1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HBA concentration was determined to be 35.93 ng/ml in human serum and undetectable in Ramos cell supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.