Mouse iNOS Recombinant Rabbit Monoclonal Antibody [PSH23-68]
cat.: HA725432
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-68 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse iNOS aa 1-506 (HA211501). |
| Positive control: | Recombinant Mouse iNOS protein (HA211501). |
| Subcellular location: | Cytoskeleton, Nucleus, Cytosol. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-67] to Mouse iNOS (Capture) (HA725431) and Recombinant Mouse iNOS protein (HA211501) as the standard. The reference range value is 19.5-5,000 pg/mL. |
| Uniprot #: | SwissProt: P29477 Mouse |
| Alternative names: | HEP-NOS Hepatocyte NOS HEPNOS inducible Inducible nitric oxide synthase Inducible NO synthase Inducible NOS iNOS MAC NOS Macrophage NOS Nitric oxide synthase 2 inducible Nitric oxide synthase 2 inducible macrophage nitric oxide synthase 2A (inducible, hepatocytes) Nitric oxide synthase Nitric oxide synthase inducible nitric oxide synthase, macrophage NOS 2 NOS Nos II NOS type II nos2 NOS2A NOS2A, Inducible, Hepatocyte Peptidyl-cysteine S-nitrosylase NOS2 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse iNOS matched pair antibodies Capture: HA725431, Mouse iNOS Rabbit mAb [PSH23-67] Detector: HA725432, Mouse iNOS Rabbit mAb [PSH23-68] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725431) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse iNOS protein (HA211501) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA725432, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native iNOS in 100 μg/ml RAW264.7 lysate samples. Capture: HA725431, Mouse iNOS Rabbit mAb [PSH23-67] Detector: HA725432, Mouse iNOS Rabbit mAb [PSH23-68] The concentrations of iNOS were measured in duplicates, interpolated from the iNOS standard curve and corrected for sample dilution. Undiluted samples are 100 μg/ml RAW264.7 lysate. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean iNOS concentration was determined to be 3,386 pg/ml in RAW264.7 lysate. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.