Mouse sTNF RI Recombinant Rabbit Monoclonal Antibody [PSH23-31]
cat.: HA725434
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-31 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Mouse sTNF RI aa 30-212 (HA211493). |
| Positive control: | Recombinant Mouse sTNF RI protein (HA211493). |
| Subcellular location: | Cell membrane, Golgi apparatus membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-30] to Mouse sTNF RI (Capture) (HA725433) and Recombinant Mouse sTNF RI protein (HA211493) as the standard. The reference range value is 7.8-2,000 pg/mL. |
| Uniprot #: | SwissProt: P25118 Mouse |
| Alternative names: | CD120a FPF MGC19588 p55 p55-R p60 TBP1 TBPI TNF R TNF R55 TNF-R1 TNF-RI TNFAR TNFR-I TNFR1 TNFR55 TNFR60 TNFRI TNFRSF1a TNR1A_HUMAN Tumor necrosis factor receptor 1 Tumor necrosis factor receptor superfamily, member 1A Tumor necrosis factor receptor type 1 Tumor necrosis factor receptor type I Tumor necrosis factor-binding protein 1 |
Images
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Fig1:
Sandwich ELISA analysis of Mouse sTNF RI matched pair antibodies Capture: HA725433, Mouse sTNF RI Rabbit mAb [PSH23-30] Detector: HA725434, Mouse sTNF RI Rabbit mAb [PSH23-31] Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725433) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse sTNF RI protein (HA211493) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725434, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native sTNF RI in mouse serum samples. Capture: HA725433, Mouse sTNF RI Rabbit mAb [PSH23-30] Detector: HA725434, Mouse sTNF RI Rabbit mAb [PSH23-31] The concentrations of sTNF RI were measured in duplicates, interpolated from the sTNF RI standard curve and corrected for sample dilution. Undiluted samples are mouse serum 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean sTNF RI concentration was determined to be 6,345 pg/ml in mouse serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.