Human TFF1 Recombinant Rabbit Monoclonal Antibody [PSH24-18]
cat.: HA725468
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-18 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TFF1 aa 25-84 (HA211418). |
| Positive control: | Recombinant Human TFF1 protein (HA211418). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-19] to Human TFF1 (Detecor) (HA725469) and Recombinant Human TFF1 protein (HA211418) as the standard. The reference range value is 15.6-2,000 pg/mL. |
| Uniprot #: | SwissProt: P04155 Human |
| Alternative names: | BCEI Breast cancer estrogen inducible protein Breast cancer estrogen inducible sequence Breast cancer estrogen-inducible protein D21S21 Gastrointestinal trefoil protein Gastrointestinal trefoil protein pS2 hP1.A HP1A HPS 2 HPS2 pNR 2 PNR-2 pNR2 Polypeptide P1.A Protein pS2 PS 2 pS2 pS2 protein TFF 1 TFF1 TFF1_HUMAN Trefoil factor 1 |
Images
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Fig1:
Sandwich ELISA analysis of Human TFF1 matched pair antibodies Capture: HA725468, Human TFF1 Rabbit mAb [PSH24-18] Detector: HA725469, Human TFF1 Rabbit mAb [PSH24-19] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725468) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TFF1 protein (HA211418) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA725469, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native TFF1 in human serum and human urine. Capture: HA725468, Human TFF1 Rabbit mAb [PSH24-18] Detector: HA725469, Human TFF1 Rabbit mAb [PSH24-19] The concentrations of TFF1 were measured in duplicates, interpolated from the TFF1 standard curve and corrected for sample dilution. Undiluted samples are human serum 50% and human urine 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TFF1 concentration was determined to be 828.93 pg/ml in human serum and 8,742.15 pg/ml in human urine. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.