Human SCGB1A1 Recombinant Rabbit Monoclonal Antibody [PSH23-84]
cat.: HA725471
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH23-84 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human SCGB1A1 aa 22-91 (HA211490). |
| Positive control: | Recombinant Human SCGB1A1 protein (HA211490). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH23-83] to Human SCGB1A1 (Capture) (HA725470) and Recombinant Human SCGB1A1 protein (HA211490) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: P11684 Human |
| Alternative names: | Blastokinin CC10 CC16 CCPBP CCSP Clara cell phospholipid binding protein Clara cell phospholipid-binding protein Clara cell specific 10 kD protein Clara cells 10 kDa secretory protein OTTHUMP00000236107 SCGB1A1 Secretoglobin family 1A member 1 Secretoglobin, family 1A, member 1 (uteroglobin) UG UGB UP-1 UP1 Urinary protein 1 Urine protein 1 UTER_HUMAN Uteroglobin |
Images
|
Fig1:
Sandwich ELISA analysis of Human SCGB1A1 matched pair antibodies Capture: HA725470, Human SCGB1A1 Rabbit mAb [PSH23-83] Detector: HA725471, Human SCGB1A1 Rabbit mAb [PSH23-84] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725470) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human SCGB1A1 protein (HA211490) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725471, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native SCGB1A1 in human plasma samples. Capture: HA725470, Human SCGB1A1 Rabbit mAb [PSH23-83] Detector: HA725471, Human SCGB1A1 Rabbit mAb [PSH23-84] The concentrations of SCGB1A1 were measured in duplicates, interpolated from the SCGB1A1 standard curve and corrected for sample dilution. Undiluted samples are 10% human plasma. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SCGB1A1 concentration was determined to be 9.6 ng/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.