Human CD38 Recombinant Rabbit Monoclonal Antibody [PSH24-45]
cat.: HA725477
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Positive control: | Recombinant Human CD38 protein (HA211487). |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-44] to Human CD38 (Capture) (HA725476) and Recombinant Human CD38 protein (HA211487) as the standard. The reference range value is 7.8-1,000 pg/mL. |
| Uniprot #: | SwissProt: P28907 Human |
| Alternative names: | Acute lymphoblastic leukemia cells antigen CD38 ADP ribosyl cyclase 1 ADP ribosyl cyclase ADP ribosyl cyclase/cyclic ADP-ribose hydrolase ADP-ribosyl cyclase 1 ADPRC 1 ADPRC1 cADPr hydrolase 1 CD 38 CD38 CD38 antigen (p45) CD38 antigen CD38 molecule Cd38-rs1 CD38_HUMAN CD38H Cyclic ADP ribose hydrolase Cyclic ADP ribose hydrolase 1 Cyclic ADP-ribose hydrolase 1 EC 3.2.2.5 Ecto nicotinamide adenine dinucleotide glycohydrolase I-19 I19 (mouse) Lymphocyte differentiation antigen CD38 NAD(+) nucleosidase NIM-R5 antigen NIMR5 antigen (mouse) OTTHUMP00000158633 OTTHUMP00000217743 p45 T10 |
Images
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Fig1:
Sandwich ELISA analysis of Human CD38 matched pair antibodies Capture: HA725476, Human CD38 Rabbit mAb [PSH24-44] Detector: HA725477, Human CD38 Rabbit mAb [PSH24-45] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725476) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CD38 protein (HA211487) starting from 1,000 pg/ml to 0 pg/ml and detect antibody (HA725477, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native CD38 in Daudi cell culture supernatant. Capture: HA725476, Human CD38 Rabbit mAb [PSH24-44] Detector: HA725477, Human CD38 Rabbit mAb [PSH24-45] Interpolated concentration of native CD38 was measured in duplicate at different sample concentrations and interpolated from the CD38 standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Daudi concentration was determined to be 2,134 pg/ml in CD38 cell culture supernatant. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.