Human APCS Recombinant Rabbit Monoclonal Antibody [PSH24-39]
cat.: HA725490
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH24-39 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human APCS aa 20-223 (HA211481). |
| Positive control: | Recombinant Human APCS protein (HA211481). |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH24-40] to Human APCS (Detecor) (HA725491) and Recombinant Human APCS protein (HA211481) as the standard. The reference range value is 19.5-10,000 pg/mL. |
| Uniprot #: | SwissProt: P02743 Human |
| Alternative names: | 9.5S alpha 1 glycoprotein 9.5S alpha-1-glycoprotein Amyloid P component serum APCS MGC88159 Pentaxin related Pentraxin 2 PTX 2 PTX2 SAMP_HUMAN SAP Serum Amyloid P Serum amyloid P component Serum amyloid P-component(1-203) |
Images
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Fig1:
Sandwich ELISA analysis of Human APCS matched pair antibodies Capture: HA725490, Human APCS Rabbit mAb [PSH24-39] Detector: HA725491, Human APCS Rabbit mAb [PSH24-40] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725490) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human APCS protein (HA211481) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA725491, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native APCS in human plasma and HepG2 supernatant. Capture: HA725490, Human APCS Rabbit mAb [PSH24-39] Detector: HA725491, Human APCS Rabbit mAb [PSH24-40] The concentrations of APCS were measured in duplicates, interpolated from the APCS standard curve and corrected for sample dilution. Undiluted samples are 0.01% human plasma and 50% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean APCS concentration was determined to be 50.6 μg/ml in human plasma. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.