Human ATG7 Recombinant Rabbit Monoclonal Antibody [PSH26-95]
cat.: HA725494
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Det) |
| Clonality: | Monoclonal |
| Clone number: | PSH26-95 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ATG7 aa 323-703 (HA211607). |
| Positive control: | Recombinant Human ATG7 protein (HA211607). |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
ELISA(Det) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-94] to Human ATG7 (Capture) (HA725493) and Recombinant Human ATG7 protein (HA211607) as the standard. The reference range value is 31.3-16,000 pg/mL. |
| Uniprot #: | SwissProt: O95352 Human |
| Alternative names: | 1810013K23Rik Apg 7 APG7 autophagy 7 like APG7 autophagy 7-like (S. cerevisiae) APG7 like APG7, S. cerevisiae, homolog of APG7-like APG7L ATG 7 ATG12-activating enzyme E1 ATG7 ATG7 ATG7 autophagy related 7 homolog (S. cerevisiae) ATG7 autophagy related 7 homolog ATG7_HUMAN Atg7l Autophagy 7, S. cerevisiae, homolog of Autophagy related protein 7 Autophagy-related 7 (yeast) Autophagy-related protein 7 DKFZp434N0735 GSA 7 GSA7 hAGP7 Ubiquitin activating enzyme E1 like protein Ubiquitin-activating enzyme E1-like protein Ubiquitin-like modifier-activating enzyme ATG7 |
Images
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Fig1:
Sandwich ELISA analysis of Human ATG7 matched pair antibodies Capture: HA725493, Human ATG7 Rabbit mAb [PSH26-94] Detector: HA725494, Human ATG7 Rabbit mAb [PSH26-95] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725493) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human ATG7 protein (HA211607) starting from 16,000 pg/mL to 0 pg/mL and detect antibody (HA725494, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native ATG7 in HeLa extract samples. Capture: HA725493, Human ATG7 Rabbit mAb [PSH26-94] Detector: HA725494, Human ATG7 Rabbit mAb [PSH26-95] The concentrations of ATG7 were measured in duplicates, interpolated from the ATG7 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ATG7 concentration was determined to be 9,296 pg/mL in HeLa extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.