Human FAP Recombinant Rabbit Monoclonal Antibody [PSH26-55]
cat.: HA725499
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH26-55 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FAP aa 26-760 (HA211419). |
| Positive control: | Recombinant Human FAP protein (HA211419). |
| Subcellular location: | Cell membrane. Cell surface. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-56] to Human FAP (Detecor) (HA725500) and Recombinant Human FAP protein (HA211419) as the standard. The reference range value is 156.3-10,000 pg/mL. |
| Uniprot #: | SwissProt: Q12884 Human |
| Alternative names: | 170 kDa melanoma membrane bound gelatinase 170 kDa melanoma membrane-bound gelatinase DPPIV FAP FAPA Fibroblast activation protein alpha Integral membrane serine protease SEPR_HUMAN Seprase |
Images
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Fig1:
Sandwich ELISA analysis of Human FAP matched pair antibodies Capture: HA725499, Human FAP Rabbit mAb [PSH26-55] Detector: HA725500, Human FAP Rabbit mAb [PSH26-56] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725499) diluted in carbonate/bicarbonate buffer, at a concentration of 5 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human FAP protein (HA211419) starting from 10,000 pg/mL to 0 pg/mL and detect antibody (HA725500, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
|
Fig2:
Interpolated concentrations of native FAP in human serum. Capture: HA725499, Human FAP Rabbit mAb [PSH26-55] Detector: HA725500, Human FAP Rabbit mAb [PSH26-56] Interpolated concentration of native FAP was measured in duplicate at different sample concentrations and interpolated from the FAP standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean FAP concentration was determined to be 69,308 pg/mL in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.