Human PDGFR alpha Recombinant Rabbit Monoclonal Antibody [PSH26-33]
cat.: HA725515
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH26-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PDGFR alpha aa 24-528 (HA211519). |
| Positive control: | Recombinant Human PDGFR alpha protein (HA211519). |
| Subcellular location: | Cell membrane, Cell projection, cilium, Golgi apparatus. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH26-34] to Human PDGFR alpha (Detecor) (HA725516) and Recombinant Human PDGFR alpha protein (HA211519) as the standard. The reference range value is 19.5-5,000 pg/mL. |
| Uniprot #: | SwissProt: P16234 Human |
| Alternative names: | Alpha-type platelet-derived growth factor receptor CD140 antigen-like family member A CD140a CD140a antigen MGC74795 PDGF alpha chain PDGF-R-alpha PDGFR 2 PDGFR alpha PDGFR2 PDGFRA PDGFRA/BCR fusion PGFRA_HUMAN Platelet derived growth factor receptor 2 Platelet derived growth factor receptor alpha Platelet derived growth factor receptor alpha polypeptide Platelet derived growth factor receptor Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein RHEPDGFRA |
Images
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Fig1:
Sandwich ELISA analysis of Human PDGFR alpha matched pair antibodies Capture: HA725515, Human PDGFR alpha Rabbit mAb [PSH26-33] Detector: HA725516, Human PDGFR alpha Rabbit mAb [PSH26-34] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725515) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human PDGFR alpha protein (HA211519) starting from 5,000 pg/mL to 0 pg/mL and detect antibody (HA725516, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native PDGFR alpha in human serum samples. Capture: HA725515, Human PDGFR alpha Rabbit mAb [PSH26-33] Detector: HA725516, Human PDGFR alpha Rabbit mAb [PSH26-34] The concentrations of PDGFR alpha were measured in duplicates, interpolated from the PDGFR alpha standard curve and corrected for sample dilution. Undiluted samples are human serum 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PDGFR alpha concentration was determined to be 5,414 pg/mL in human serum. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.