Human TNFSF14 Recombinant Rabbit Monoclonal Antibody [PSH27-26]
cat.: HA725533
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA(Cap) |
| Clonality: | Monoclonal |
| Clone number: | PSH27-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TNFSF14 aa 74-240 (HA211392). |
| Positive control: | Recombinant Human TNFSF14 protein (HA211392). |
| Subcellular location: | Cell membrane, Secreted, Cytoplasm. |
| Recommended Dilutions:
ELISA(Cap) |
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH27-27] to Human TNFSF14 (Detecor) (HA725534) and Recombinant Human TNFSF14 protein (HA211392) as the standard. The reference range value is 3.9-1,000 pg/mL. |
| Uniprot #: | SwissProt: O43557 Human |
| Alternative names: | CD 258 CD258 CD258 antigen Delta transmembrane LIGHT Herpes virus entry mediator ligand Herpesvirus entry mediator A Herpesvirus entry mediator ligand herpesvirus entry mediator-ligand HVEM L HVEM-L HVEML Ligand for herpesvirus entry mediator LIGHT LTg soluble form TNF14 TNF14_HUMAN TNFSF 14 Tnfsf14 TNFSF14 protein TR 2 TR2 Tumor necrosis factor (ligand) superfamily, member 14 Tumor necrosis factor ligand superfamily member 14 Tumor necrosis factor ligand superfamily, member 14 Tumor necrosis factor receptor like 2 tumor necrosis factor receptor-like 2 Tumor necrosis factor superfamily member 14 Tumor necrosis factor superfamily member LIGHT |
Images
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Fig1:
Sandwich ELISA analysis of Human TNFSF14 matched pair antibodies Capture: HA725533, Human TNFSF14 Rabbit mAb [PSH27-26] Detector: HA725534, Human TNFSF14 Rabbit mAb [PSH27-27] Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA725533) diluted in carbonate/bicarbonate buffer, at a concentration of 2 μg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human TNFSF14 protein (HA211392) starting from 1,000 pg/mL to 0 pg/mL and detect antibody (HA725534, Biotin, 0.2 μg/mL) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. |
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Fig2:
Interpolated concentrations of native TNFSF14 in KG-1 and MCF7 cell extract samples based on 1 mg/mL extract loading. Capture: HA725533, Human TNFSF14 Rabbit mAb [PSH27-26] Detector: HA725534, Human TNFSF14 Rabbit mAb [PSH27-27] The concentrations of TNFSF14 were measured in duplicates, interpolated from the TNFSF14 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean TNFSF14 concentration was determined to be 385 pg/mL in KG-1 cell extract. There was no detectable signal in MCF7 cell extract. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.