Insulin Recombinant Rabbit Monoclonal Antibody [SA0410]
cat.: HA750004
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IF-Tissue, IHC-P, IHC-Fr, WB |
| Clonality: | Monoclonal |
| Clone number: | SA0410 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Insulin aa 15-110. |
| Positive control: | Mouse pancreas tissue, human pancreas tissue, rat pancreas tissue, Mouse pancreas tissue lysate, Rat pancreas tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IF-Cell IF-Tissue IHC-P IHC-Fr WB |
1:200-1:500 1:200-1:500 1:20,000 1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P01308 Human | P01325 Mouse | P01322 Rat |
| Alternative names: | IDDM IDDM1 IDDM2 ILPR ins INS_HUMAN Insulin A chain Insulin B chain IRDN MODY10 Preproinsulin Proinsulin Proinsulin precursor |
Images
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Fig1:
Immunofluorescence analysis of paraffin-embedded mouse pancreas tissue labeling Insulin with Rabbit anti-Insulin antibody (HA750004) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750004, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Insulin with Rabbit anti-Insulin antibody (HA750004) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750004, red) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as the secondary antibody at 1/500 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig3:
Immunofluorescence analysis of paraffin-embedded human pancreas tissue labeling Insulin (HA750004) and beta III Tubulin (M0805-8). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Insulin (HA750004, red) at 1/200 dilution and beta III Tubulin (M0805-8, green) at 1/200 dilution at +4℃ overnight, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) and Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-Insulin antibody (HA750004) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750004) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-Insulin antibody (HA750004) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750004) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-Insulin antibody (HA750004) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750004) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunofluorescence analysis of frozen mouse pancreas tissue with Rabbit anti-Insulin antibody (HA750004) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750004, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig8:
Western blot analysis of Insulin on different lysates with Rabbit anti-Insulin antibody (HA750004) at 1/1,000 dilution. Lane 1: Mouse pancreas tissue lysate Lane 2: Rat pancreas tissue lysate Lane 3: Rat colon tissue lysate (negative) Lysates/proteins at 40 µg/Lane. Predicted band size: 12 kDa Observed band size: 12 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750004) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.