Alkaline Phosphatase Recombinant Rabbit Monoclonal Antibody [SA40-00]
cat.: HA750013
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | SA40-00 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Alkaline Phosphatase aa 18-50. |
| Positive control: | Saos-2 cell lysate, HeLa cell lysate, A549 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, human liver tissue, mouse liver tissue, rat liver tissue, mouse jawbone tissue. |
| Subcellular location: | Cell membrane, Mitochondrion membrane, Mitochondrion intermembrane space, Extracellular vesicle membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:5,000 1:1,000-1:8,000 1:200 |
| Uniprot #: | SwissProt: P05186 Human | P09242 Mouse | P08289 Rat |
| Alternative names: | Alkaline phosphatase Alkaline phosphatase placental Alkaline phosphatase placental type Alkaline phosphatase Regan isozyme ALP Alp1 ALPP FLJ61142 Germ-cell alkaline phosphatase nagao isozyme OTTHUMP00000164354 PALP Placental alkaline phosphatase 1 placental heat-stable alkaline phosphatase placental type PLAP PLAP-1 PLAP1 PPB1_HUMAN |
Images
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Fig1:
Western blot analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody (HA750013) at 1/5,000 dilution. Lane 1: Saos-2 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: A549 cell lysate (15 µg/Lane) Lane 4: Mouse liver tissue lysate (20 µg/Lane) Lane 5: Rat liver tissue lysate (20 µg/Lane) Predicted band size: 57 kDa Observed band size: 75 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750013) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alkaline Phosphatase antibody (HA750013) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750013) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alkaline Phosphatase antibody (HA750013) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750013) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Alkaline Phosphatase antibody (HA750013) at 1/8,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750013) at 1/8,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse jawbone tissue with Rabbit anti-Alkaline Phosphatase antibody (HA750013) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750013) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Application: Immunohistochemistry (IHC-P) Species: Mouse Tissue: Alveolar bone Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: HA750013, 1/5,000, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
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Fig7:
Application: IF-Tissue Species: Human Site: liver Sample: Paraffin-embedded section Antibody concentration: 1/200 |
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Fig8:
Application: IF-Tissue Species: Mouse Site: bone Sample: Paraffin-embedded section Antibody concentration: 1/200 |
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Fig9:
Application: IF-Tissue Species: Mouse Site: bone Sample: Paraffin-embedded section Antibody concentration: 1/200 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.