LDL Receptor Recombinant Rabbit Monoclonal Antibody [SJ0197]
cat.: HA750107
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC
Clonality: Monoclonal
Clone number: SJ0197
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4).
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 95 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human LDL Receptor aa 811-860 / 860.
Positive control: HEK-293 cell lysate, Huh7 cell lysate, NIH/3T3 cell lysate, Mouse liver tissue lysate, Rat liver tissue lysate, PC-12 cell lysates, A549, HeLa.
Subcellular location: Cell membrane, Membrane, clathrin-coated pit, Golgi apparatus, Early endosome, Late endosome, Lysosome.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:1,000-1:5,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P01130 Human | P35951 Mouse | P35952 Rat
Alternative names: FH FHC LDL R LDL receptor LDLCQ2 Ldlr LDLR_HUMAN Low Density Lipoprotein Receptor Low density lipoprotein receptor class A domain containing protein 3 Low density lipoprotein receptor familial hypercholesterolemia Low-density lipoprotein receptor
Images
HA750107_1.jpg Fig1: Western blot analysis of LDL Receptor on different lysates with Rabbit anti-LDL Receptor antibody (HA750107) at 1/5,000 dilution.

Lane 1: HEK-293 cell lysate (low expression) (20 µg/Lane)
Lane 2: Huh7 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse liver tissue lysate (30 µg/Lane)
Lane 5: Rat liver tissue lysate (30 µg/Lane)

Predicted band size: 95 kDa
Observed band size: 150 kDa

Exposure time: 3 minutes; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750107) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA750107_2.jpg Fig2: Western blot analysis of LDL Receptor on PC-12 cell lysates with Rabbit anti-LDL Receptor antibody (HA750107) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 95 kDa
Observed band size: 140 kDa

Exposure time: 6 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750107) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA750107_3.jpg Fig3: Immunocytochemistry analysis of A549 cells labeling LDL Receptor with Rabbit anti-LDL Receptor antibody (HA750107) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LDL Receptor antibody (HA750107) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA750107_4.jpg Fig4: Flow cytometric analysis of HeLa cells labeling LDL Receptor.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA750107, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.