Aurora B Recombinant Rabbit Monoclonal Antibody [SC55-08]
cat.: HA750208
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IP, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | SC55-08 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Aurora B aa 1-50 / 344. |
| Positive control: | Hela cell lysate, MCF-7 cell lysate, human tonsil tissue, HeLa. |
| Subcellular location: | Nucleus, Cytoplasm, Chromosome. |
| Recommended Dilutions:
WB IHC-P IF-Cell IP |
1:1,000-1:2,000 1:50-1:200 1:100 1-2μg/sample |
| Uniprot #: | SwissProt: Q96GD4 Human | O70126 Mouse | O55099 Rat |
| Alternative names: | AIK2 AIM-1 AIM1 ARK-2 ARK2 AurB AURKB AURKB_HUMAN Aurora 1 Aurora and Ipl1 like midbody associated protein 1 Aurora kinase B Aurora related kinase 2 Aurora- and Ipl1-like midbody-associated protein 1 Aurora-B Aurora-related kinase 2 Aurora/IPL1 related kinase 2 Aurora/IPL1-related kinase 2 IPL1 PPP1R48 Protein phosphatase 1 regulatory subunit 48 Serine/theronine kinase 12 Serine/threonine protein kinase 12 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase aurora-B STK-1 STK1 STK12 STK5 |
Images
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Fig1:
Western blot analysis of Aurora B on different lysates using anti-Aurora B antibody at 1/1,000 dilution. Positive control: Lane 1: Hela cell lysates Lane 2: MCF-7 cell lysates |
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Fig2:
All lanes: Western blot analysis of Aurora B with anti-Aurora B antibody (HA750208) at 1:500 dilution. Lane 1: Wild-type Hela whole cell lysate (10 µg). Lane 2: Aurora B knockdown Hela whole cell lysate (10 µg). ET1610-25 was shown to specifically react with Aurora B in wild-type Hela cells. Weakened band was observed when Aurora B knockdown sample was tested. Wild-type and Aurora B knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1610-25, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Aurora B antibody. Counter stained with hematoxylin. |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling Aurora B with Rabbit anti-Aurora B antibody (HA750208) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Aurora B antibody (HA750208) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5:
Aurora B was immunoprecipitated from 0.2 mg HepG2 cell lysate with HA750208 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750208 at 1/1,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HepG2 cell lysate (input) Lane 2: HA750208 IP in HepG2 cell lysate Lane 3: Rabbit IgG instead of HA750208 in HepG2 cell lysate Blocking/Dilution buffer: primary antibody dilution (K1803) Exposure time: 20 seconds; ECL: K1801 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.