Mast Cell Tryptase Recombinant Rabbit Monoclonal Antibody [SC68-07]
cat.: HA750229
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | SC68-07 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Mast Cell Tryptase aa 10-275 / 275. |
| Positive control: | Human lung tissue lysates, mouse skin tissue lysates, human lung tissue, human tonsil tissue, human small intestine tissue, human prostate carcinoma tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:5,000-1:10,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: Q15661 Human | Q02844 Mouse |
| Alternative names: | alpha II Lung tryptase Mast cell alpha II tryptase Mast cell beta I tryptase Mast cell protease 7 Mast cell protease II MCP 7 Pituitary tryptase Skin tryptase TPS 1 TPS1 TPS2 TPSAB1 TPSAB1 protein TPSB1 Tryptase 1 Tryptase alpha 1 tryptase alpha I included Tryptase alpha II tryptase alpha II included tryptase alpha included tryptase alpha/beta 1 Tryptase beta 1 tryptase beta I included Tryptase I tryptase I included Tryptase III Tryptase skin |
Images
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Fig1:
Western blot analysis of Mast Cell Tryptase on different lysates with Rabbit anti-Mast Cell Tryptase antibody (HA750229) at 1/1,000 dilution. Lane 1: Human lung tissue lysate (40 µg/Lane) Lane 2: Mouse skin tissue lysate (40 µg/Lane) Predicted band size: 30 kDa Observed band size: 35 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750229) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Mast Cell Tryptase antibody (HA750229) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750229) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.