Tyrosine Hydroxylase Recombinant Rabbit Monoclonal Antibody [SN59-03]
cat.: HA750243
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey |
| Applications: | WB, IF-Cell, IHC-P, FC, IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | SN59-03 |
| Form: | Liquid |
| Storage condition: | Store at 2-8℃. Avoid freeze. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 59 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Tyrosine Hydroxylase. |
| Positive control: | Rat brain tissue lysates, PC-12 cell lysates, N2A, SH-SY5Y, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm. |
| Uniprot #: | SwissProt: P07101 Human | P24529 Mouse | P04177 Rat |
| Alternative names: | Dystonia 14 DYT14 DYT5b EC 1.14.16.2 OTTHUMP00000011225 OTTHUMP00000011226 ple Protein Pale TH The TY3H_HUMAN TYH Tyrosine 3 hydroxylase Tyrosine 3 monooxygenase Tyrosine 3-hydroxylase Tyrosine 3-monooxygenase Tyrosine hydroxylase |
Images
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Fig1:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Immunofluorescence analysis of frozen mouse brain tissue with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750243, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig2:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750243) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750243) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Western blot analysis of Tyrosine Hydroxylase on PC-12 cell lysates with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Predicted band size: 59 kDa Observed band size: 59 kDa Exposure time: 24 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750243) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig5:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling Tyrosine Hydroxylase with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750243, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig6:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Tyrosine Hydroxylase with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750243, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
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Fig7:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Immunocytochemistry analysis of N2A cells labeling Tyrosine Hydroxylase with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig8:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Immunocytochemistry analysis of SH-SY5Y cells labeling Tyrosine Hydroxylase with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig9:
This data was developed using HA750243, the same antibody clone in a different buffer formulation. Western blot analysis of Tyrosine Hydroxylase on different lysates with Rabbit anti-Tyrosine Hydroxylase antibody (HA750243) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA750243, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59 kDa Observed band size: 55 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.