HA750337

Myelin Basic Protein Recombinant Rabbit Monoclonal Antibody [JF0943]

cat.: HA750337

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	WB, IHC-P, IF-Tissue, IHC-Fr
Clonality:	Monoclonal
Clone number:	JF0943

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4).
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 33 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human Myelin Basic Protein aa 121-304 / 304.
Positive control:	Rat brain tissue lysate, Mouse brain tissue lysate, human brain tissue, mouse brain tissue, rat brain tissue, mouse cerebral cortex tissue, mouse hippocampus tissue.
Subcellular location:	Myelin membrane, Nucleus.
Recommended Dilutions: WB IHC-P IF-Tissue IHC-Fr	1:1,000 1:1,000-1:30,000 1:500-1:1,000 1:500-1:1,000
Uniprot #:	SwissProt: P02686 Human \| P04370 Mouse \| P02688 Rat
Alternative names:	GDB Golli MBP Golli MBP; myelin basic protein Hemopoietic MBP HMBPR HUGO MBP MBP_CAVPO MBP_HUMAN MGC99675 MLD Myelin A1 protein Myelin A1 Protein, basic Myelin basic protein Myelin Deficient Myelin membrane encephalitogenic protein OTTHUMP00000163776 OTTHUMP00000174387 OTTHUMP00000174388 SHI Shiverer SP

Images

	Fig1: Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required
	Fig2: Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:1,000 Antigen retrieval: Not required
	Fig3: Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required
	Fig4: Application: IF-tissue Species: Human Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1:500

	Fig5: Application: IF-tissue Species: Mouse Site: Hippocampus Sample: Paraffin-embedded section Antibody concentration: 1:500
	Fig6: Western blot analysis of Myelin Basic Protein on different lysates with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 33 kDa Observed band size: 14~25 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750337) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
	Fig7: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750337) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue (negative) with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750337) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/30,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750337) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig10: Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750337) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Fig11: Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Myelin Basic Protein antibody (HA750337) at 1/30,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750337) at 1/30,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.