Calbindin Recombinant Rabbit Monoclonal Antibody [JF05-01]
cat.: HA750351
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IF-Tissue, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JF05-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 30 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Calbindin aa 51-100 / 261. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, human kidney tissue, mouse cerebellum tissue, PC-12, 293T, rat brain tissue, mouse brain tissue, mouse kidney tissue. |
| Subcellular location: | Cytosol, Nucleus. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IHC-Fr |
1:1,000-1:5,000 1:500 1:5,000-1:10,000 1:2,000 |
| Uniprot #: | SwissProt: P05937 Human | P12658 Mouse | P07171 Rat |
| Alternative names: | avian-type CAB27 CALB 1 CALB CALB1 CALB1_HUMAN Calbindin 1 28kDa Calbindin Calbindin D28 D 28K D-28K D28K OTTHUMP00000166027 OTTHUMP00000225441 RTVL H protein Vitamin D dependent calcium binding protein Vitamin D dependent calcium binding protein avian type Vitamin D-dependent calcium-binding protein |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/2,000 Antigen retrieval: Not required |
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Fig2:
Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig3:
Application: IF-tissue Species: Rat Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500 |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Calbindin antibody (HA750351) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750351) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Calbindin antibody (HA750351) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750351) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Western blot analysis of Calbindin on different lysates with Rabbit anti-Calbindin antibody (HA750351) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: NIH/3T3 cell lysate (negative) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: C6 cell lysate (negative) (20 µg/Lane) Lane 5: Mouse kidney tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 30 kDa Observed band size: 30 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750351) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.