HA750371

Parvalbumin Recombinant Rabbit Monoclonal Antibody [JM100-08]

cat.: HA750371

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	WB, IHC-P, IHC-Fr, IF-Tissue, IP
Clonality:	Monoclonal
Clone number:	JM100-08

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4).
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 12 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within Human Paravalbumin aa 1-110 / 110.
Positive control:	Mouse cerebellum tissue, rat cerebellum tissue, human cerebellum tissue, RPMI 8226 cell lysate, Mouse skeletal muscle tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.
Subcellular location:	Axon, cytoplasm, synapse.
Recommended Dilutions: WB IHC-P IHC-Fr IF-Tissue IP	1:1,000 1:5,000-1:50,000 1:500-1:1,000 1:500 1-2μg/sample
Uniprot #:	SwissProt: P20472 Human \| P32848 Mouse \| P02625 Rat
Alternative names:	D22S749 MGC116759 Parvalbumin alpha PRVA_HUMAN PVALB

Images

	Fig1: Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Parvalbumin, HA750371, red); 1/500 (SLC32A1 / VGAT, HA601384, green) Antigen retrieval: Not required
	Fig2: Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (Parvalbumin, HA750371, red); 1/500 (SLC32A1 / VGAT, HA601384, green) Antigen retrieval: Not required
	Fig3: Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required

	Fig4: Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/50,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750371) at 1/50,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750371) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig7: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750371) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Parvalbumin antibody (HA750371) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750371) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig9: Western blot analysis of Parvalbumin on different lysates with Rabbit anti-Parvalbumin antibody (HA750371) at 1/1,000 dilution. Lane 1: RPMI 8226 cell lysate (20 µg/Lane) Lane 2: SK-MEL-28 cell lysate (negative) (20 µg/Lane) Lane 3: Mouse skeletal muscle tissue lysate (20 µg/Lane) Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane) Lane 5: Rat cerebellum tissue lysate (40 µg/Lane) Predicted band size: 12 kDa Observed band size: 14 kDa Exposure time: Lane 1-3: 30 seconds; Lane 4-5: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig10: Application: IF-tissue

Species: Mouse

Site: Cerebellum

Sample: Paraffin-embedded section

Antibody concentration: 1/500

Fig11: Parvalbumin was immunoprecipitated from 0.2 mg RPMI 8226 cell lysate with HA750371 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750371 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

Lane 1: RPMI 8226 cell lysate (input)
Lane 2: HA750371 IP in RPMI 8226 cell lysate
Lane 3: Rabbit IgG instead of HA750371 in RPMI 8226 cell lysate

Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 10 seconds; ECL: K1801

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.