HA750378

AQP1 Recombinant Rabbit Monoclonal Antibody [JM10-98]

cat.: HA750378

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat, Cynomolgus monkey, Pig
Applications:	WB, IF-Tissue, IHC-P
Clonality:	Monoclonal
Clone number:	JM10-98

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4).
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 28 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human AQP1 aa 245-269 / 269.
Positive control:	Human lung tissue lysate, rat kidney tissue lysate, rat lung tissue lysate, mouse kidney tissue lysate, mouse lung tissue lysate, MCF-7, Hela, SW480, human spleen tissue, human kidney tissue, human pancreas tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IF-Tissue IHC-P	1:2,000-1:5,000 1:500-1:2,000 1:5,000
Uniprot #:	SwissProt: P29972 Human \| Q02013 Mouse \| P29975 Rat
Alternative names:	AQP 1 AQP CHIP AQP-1 AQP1 AQP1_HUMAN aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group) aquaporin 1 (Colton blood group) Aquaporin CHIP Aquaporin-1 Aquaporin-CHIP Aquaporin1 Channel forming integral protein 28kDa Channel like integral membrane protein 28 kDa CHIP 28 CHIP28 CO Colton blood group Growth factor induced delayed early response protein MGC26324 Urine water channel Water channel protein CHIP 29 Water channel protein CHIP29 Water channel protein for red blood cells and kidney proximal tubule

Images

Fig1: Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (HA750378) at 1/2,000 dilution.

Lane 1: Human lung tissue lysate
Lane 2: Rat kidney tissue lysate
Lane 3: Rat kidney tissue lysate (no heat)
Lane 4: Rat lung tissue lysate
Lane 5: Rat lung tissue lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 28~40 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of AQP1 on different lysates with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution.

Lane 1: Mouse kidney tissue lysate
Lane 2: Mouse kidney tissue lysate (no heat)
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse lung tissue lysate (no heat)

Notice: no heat means the lysate is not boiled.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 28~40 kDa
Exposure time: 24 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750378) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-AQP1 antibody (HA750378) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA750378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Application: IF-Tissue Species: Mouse Site: kidney Sample: Paraffin-embedded section Antibody concentration: 1/1,000

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.