MHC Class II Recombinant Rabbit Monoclonal Antibody [JA10-94]
cat.: HA750404
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IF-Cell, IF-Tissue, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JA10-94 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HLA-DPB1 aa 11-60 / 258. |
| Positive control: | Daudi cell lysates, human tonsil tissue, human endometrium tissue, Daudi. |
| Subcellular location: | Cell membrane. Endoplasmic reticulum membrane. |
| Recommended Dilutions:
WB IF-Cell IF-Tissue IHC-P IP |
1:5,000 1:50 1:50-1:100 1:1,000 Use at an assay dependent concentration. |
| Uniprot #: | SwissProt: P04440 Human |
| Alternative names: | D6S221E DMA DMB DP beta 1 DP beta 1 chain DP(W4) beta chain DPB 1 DPB1 DPB1_HUMAN DRB H2Ea HLA class II histocompatibility antigen HLA class II histocompatibility antigen DM beta chain HLA class II histocompatibility antigen, DP beta 1 chain HLA class II histocompatibility antigen, DP(W4) beta chain HLA DMB HLA DP1A HLA DPB1 HLA SB alpha chain HLA-A HLA-A histocompatibility type HLA-DP HLA-DP histocompatibility type, beta-1 subunit HLA-DP1B HLA-DPB HLA-DPB1 HLADM HLADP1B HLASB HLASB histocompatibility type Human MHC class II HLA SB alpha LA class II histocompatibility antigen DP alpha 1 chain Major histocompatibility complex class II Major histocompatibility complex class II DP alpha 1 Major histocompatibility complex class II DP beta 1 Major histocompatibility complex, class I, A MHC class II antigen DMB MHC class II antigen DPB1 MHC class II DP3 alpha MHC class II DPA1 MHC class II HLA-DP-beta-1 MHC DPB...... |
Images
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Fig1:
Western blot analysis of MHC Class II on Daudi cell lysates with Rabbit anti-MHC Class II antibody (HA750404) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750404) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-MHC Class II antibody (HA750404) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750404) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-MHC Class II antibody (HA750404) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750404) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Daudi cells labeling MHC Class II with Rabbit anti-MHC Class II antibody (HA750404) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MHC Class II antibody (HA750404) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.