FAP Recombinant Rabbit Monoclonal Antibody [JA56-11]
cat.: HA750409
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | JA56-11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 88 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human FAP1 aa 1-160 / 760. |
| Positive control: | U-87 MG cell lysates, human pancreatic carcinoma, human colon cancer tissue. |
| Subcellular location: | Cell membrane. Cell surface. |
| Recommended Dilutions:
WB IHC-P IHC-Fr |
1:1,000 1:1,000 1:200 |
| Uniprot #: | SwissProt: Q12884 Human | P97321 Mouse | Q8R492 Rat |
| Alternative names: | 170 kDa melanoma membrane bound gelatinase 170 kDa melanoma membrane-bound gelatinase DPPIV FAP FAPA Fibroblast activation protein alpha Integral membrane serine protease SEPR_HUMAN Seprase |
Images
|
Fig1:
Western blot analysis of FAP on U-87 MG cell lysates with Rabbit anti-FAP antibody (HA750409) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 88 kDa Observed band size: 100 kDa Exposure time: 5 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750409) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-FAP antibody (HA750409) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750409) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunofluorescence analysis of frozen mouse colon tissue with Rabbit anti-FAP antibody (HA750409) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750409, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
|
Fig4:
Immunofluorescence analysis of frozen rat colon tissue with Rabbit anti-FAP antibody (HA750409) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for about 2 minutes in microwave oven. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750409, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.