Villin1 Recombinant Rabbit Monoclonal Antibody [A2]
cat.: HA750477
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | A2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Villin1 aa 600-827. |
| Positive control: | HT-29 (Human colorectal adenocarcinoma cell) cell lysate, Caco-2 (Human colorectal adenocarcinoma cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, human kidney tissue, human small intestine tissue. |
| Subcellular location: | Cytoskeleton, lamellipodium, ruffle, microvillus, filopodium tip, filopodium. |
| Recommended Dilutions:
WB IHC-P |
1:10,000 1:3,000 |
| Uniprot #: | SwissProt: P09327 Human |
| Alternative names: | D2S1471 OTTHUMP00000164145 VIL VIL1 VILI_HUMAN Villin 1 Villin-1 Villin1 |
Images
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Fig1:
Western blot analysis of Villin1 on different lysates with Rabbit anti-Villin1 antibody (HA750477) at 1/10,000 dilution. Lane 1: HT-29 (Human colorectal adenocarcinoma cell) cell lysate Lane 2: Caco-2 (Human colorectal adenocarcinoma cell) cell lysate Lane 3: Hep G2 (Human hepatocellular carcinoma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 17 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA750477, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 93 kDa Observed band size: 93 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Villin1 antibody (HA750477) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750477) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Villin1 antibody (HA750477) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750477) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.