hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-01]
cat.: HA750554
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PH00-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 186 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C terminal human SET1A. |
| Positive control: | HeLa cell lysate,293T cell lysate, Jurkat cell lysate, HepG2 cell lysate,human lung carcinoma tissue, human testis tissue, mouse hippocampus tissue. |
| Subcellular location: | Chromosome, Nucleus |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:400 |
| Uniprot #: | SwissProt: O15047 Human | E9PYH6 Mouse | A0A8I6AS32 Rat |
| Alternative names: | Histone-lysine N-methyltransferase SETD1A hSET1A KMT2F Lysine N methyltransferase 2F Lysine N-methyltransferase 2F SET domain containing 1A SET domain containing protein 1A SET domain-containing protein 1A SET1 Set1 Ash2 histone methyltransferase complex subunit SET1 Set1/Ash2 histone methyltransferase complex subunit SET1 SET1A SET1A_HUMAN SETD1A |
Images
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Fig1:
Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 4: HepG2 (Human liver cancer cells) cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 90 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA750554, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/200,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 186 kDa Observed band size: 250~300 kDa |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750554) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750554) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-hSET1/SET1 antibody (HA750554) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750554) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.