hSET1/SET1 Recombinant Rabbit Monoclonal Antibody [PH00-02]
cat.: HA750556
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PH00-02 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 186 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C terminal human SET1A. |
| Positive control: | 293T cell lysates, 293T, HepG2. |
| Subcellular location: | Chromosome, Nucleus |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: O15047 Human | E9PYH6 Mouse | A0A8I6AS32 Rat |
| Alternative names: | Histone-lysine N-methyltransferase SETD1A hSET1A KMT2F Lysine N methyltransferase 2F Lysine N-methyltransferase 2F SET domain containing 1A SET domain containing protein 1A SET domain-containing protein 1A SET1 Set1 Ash2 histone methyltransferase complex subunit SET1 Set1/Ash2 histone methyltransferase complex subunit SET1 SET1A SET1A_HUMAN SETD1A |
Images
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Fig1:
Flow cytometric analysis of 293T cells labeling hSET1/SET1. Cells were fixed and permeabilized.Then stained with the primary antibody (HA750556, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig2:
Flow cytometric analysis of HepG2 cells labeling hSET1/SET1. Cells were fixed and permeabilized.Then stained with the primary antibody (HA750556, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Western blot analysis of hSET1/SET1 on different lysates with Rabbit anti-hSET1/SET1 antibody (HA750556) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: HEK 293T cell lysate Lane 3: Jurkat (Human T-lymphoblastic cells) cell lysate Lane 4: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 30 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA750556, 1/1,000 in primary antibody, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 186.0 kDa Observed band size: 250~300 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.