ASC1 Recombinant Rabbit Monoclonal Antibody [PSH0-26]
cat.: HA750600
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH0-26 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 66 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ASC1 aa 1-581 / 581. |
| Positive control: | A549 cell lysate, Hela cell lysate, U87-MG cell lysate, 293T cell lysate, HL-60 cell lysate, HepG2 cell lysate, RH-35 cell lysate, HeLa cell lysate, Rat testis tissue lysate, rat small intestine tissue. |
| Subcellular location: | Nucleus, Cytoplasm, cytosol, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q15650 Human Entrez Gene: 315769 Rat |
| Alternative names: | Activating signal cointegrator 1 ASC 1 ASC-1 HsT17391 Thyroid hormone receptor interactor 4 Thyroid receptor interacting protein 4 Thyroid receptor-interacting protein 4 TR-interacting protein 4 TRIP 4 TRIP-4 Trip4 TRIP4_HUMAN ZC2HC5 Zinc finger C2HC5 type |
Images
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Fig1:
Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA750600) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: Hela cell lysate Lane 3: U87-MG cell lysate Lane 4: 293T cell lysate Lane 5: HL-60 cell lysate Lane 6: HepG2 cell lysate Lane 7: RH-35 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 3 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750600) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of ASC1 on different lysates with Rabbit anti-ASC1 antibody (HA750600) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: Rat testis tissue lysate (40 µg/Lane) Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750600) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Rabbit anti-ASC1 antibody (HA750600) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750600) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.