Cystathionase Recombinant Rabbit Monoclonal Antibody [JE35-91]
cat.: HA750649
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | JE35-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 45 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Cystathionase aa 1-405 / 405. |
| Positive control: | HepG2 cell lysate, 293T cell lysate, K-562 cell lysate, Human liver tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate,. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IP |
1:1,000-1:10,000 1-2μg/sample |
| Uniprot #: | SwissProt: P32929 Human | Q8VCN5 Mouse | P18757 Rat |
| Alternative names: | CGL_HUMAN CTH cystathionase (cystathionine gamma-lyase) Cystathionine gamma lyase Cystathionine gamma-lyase Cysteine desulfhydrase Gamma cystathionase Gamma-cystathionase Homoserine deaminase Homoserine dehydratase MGC9471 |
Images
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Fig1:
Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA750649) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: 293T cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: Human liver tissue lysate (40 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750649) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Cystathionase was immunoprecipitated in 0.2mg A431 cell lysate with HA750649 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA750649 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: A431 cell lysate (input) Lane 2: HA750649 IP in A431 cell lysate Lane 3: Rabbit IgG instead of HA750649 in A431 cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds |
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Fig3:
Western blot analysis of Cystathionase on different lysates with Rabbit anti-Cystathionase antibody (HA750649) at 1/10,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Cystathionase KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750649) at 1/10,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.