CTU2 Recombinant Rabbit Monoclonal Antibody [PSH04-05]
cat.: HA750928
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Monkey |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | PSH04-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4). |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CTU2 aa 1-515 / 515. |
| Positive control: | HeLa cell lysate, HT-29 cell lysate, 293T cell lysate, K-562 cell lysate, Raji cell lysate, HepG2 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, mouse spleen tissue lysate, mouse liver tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:2,000 1:200 |
| Uniprot #: | SwissProt: Q2VPK5 Human | Q3U308 Mouse |
| Alternative names: | C16orf84 ctu2 CTU2_HUMAN Cytoplasmic tRNA 2-thiolation protein 2 cytosolic thiouridylase subunit 2 homolog (S. pombe) NCS2 PF0432 |
Images
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Fig1:
Western blot analysis of CTU2 on different lysates with Rabbit anti-CTU2 antibody (HA750928) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HT-29 cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: Raji cell lysate Lane 6: HepG2 cell lysate Lane 7: COS-1 cell lysate Lane 8: NIH/3T3 cell lysate Lane 9: Mouse spleen tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 56 kDa Observed band size: 54/56 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750928) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-CTU2 antibody (HA750928) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750928) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.